Biological Inorganic Chemistry, Chemical Biology

1. Design and construction of fluorescent biosensors and molecular logic gate, based on nucleic acid aptamer, antibody / antigen, combined with quantum dots, graphene nano materials, targeting to the applications of clinical diagnosis, environmental monitoring and food safety control;

2. Innovative stratagem for searching molecular architecture of tau aggregation inhibitor, screening lead compounds for the therapy of Alzheimer’s disease (AD). Natural products and their derivatives were introduced as the references that integrates tau binding properties and inhibitory effects. A two-side recognition, hairpin-like molecular framework was suggested by us;

3. Rational design and synthesis of novel Ruthenium complex as the molecular probe for protein conformation alteration and genetic variation. Exploring the interaction of metal complexes with disease-related proteins / nucleic acids, revealing the pathogenic mechanisms of diseases such as AD and cancer, at molecular level.

1. Ruru Gao, Shuo Shi*, Ying Zhu, Hailiang Huang, Tianming Yao*, A RET-supported logic gate combinatorial library to enable modeling and implementation of intelligent logic functions,Chemical Science, 2016, 7, 1853-1861.

2. Ying Zhu, Xiaochun Hu, Shuo Shi*, Ruru Gao, Hailiang Huang, Yanyan Zhu, Xiaoyan Lv, Tianming Yao*, Ultrasensitive and universal fluorescent aptasensor for the detection of biomolecules (ATP,adenosine and thrombin)based on DNA/Ag nanoclusters, Biosensors and Bioelectronics, 2016, 79, 205-212.

3. Hailiang Huang, Shuo Shi*, Xing Gao, Ruru Gao, Ying Zhu, Xuewen Wu, Ruimin Zan, Tianming Yao*, A universall abel-free fluorescent aptasensor, based on Ru complex and quantum dots for adenosine, dopamine and 17β-estradiol detection, Biosensors and Bioelectronics, 2016, 79, 198-204

4. Juanjuan Li, Xiaochun Hu, Shuo Shi*, Yiwei Zhang, Tianming Yao*, Three label-free thrombin aptasensors based on aptamers and [Ru(bpy)₂(o-mopip)]²⁺, Journal of Materials Chemistry B,2016, 4, 1361-1367.

5. Hailiang Huang, Shuo Shi*, Xuyue Zhen, Tianming Yao*, Sensitive detection for coralyne and mercury ions based on homo-A/T DNA by exonuclease signa lamplification, Biosensors and Bioelectronics, 2015, 71, 439-444.

6. Shuo Shi*, Jinhong Xu, Xing Gao, Hailiang Huang, Tianming Yao*, Binding Behaviors for Different Types of DNA G-Quadruplexes:，Enantiomers of [Ru(bpy)₂(L)]²⁺ (L=dppz, dppz-idzo),CHEMISTRY A European Journal, 2015, 21, 11435-11445.

7. Xuyue Zhen, Tianming Yao*, Ying Zhu, Shuo Shi*, Cu²⁺ modulated silver nanoclusters as an on–off–on fluorescence probe for the selective detection of L-histidine, Biosensors and Bioelectronics, 2015, 66, 103-108.

8. Linlin Wang, Tianming Yao*, Shuo Shi*, Yanlin Cao, Wenliang Sun, A label-free fluorescent probe for Hg 2+ and biothiols based on graphene oxide and Ru-complex, Scientific Reports, 2014, 4, 5320.

9. Yanlin Cao, Shuo Shi*, Linlin Wang, Junliang Yao, Tianming Yao*, Ultrasensitive fluorescence detection of heparin based on quantum dots and a functional ruthenium polypyridyl complex,Biosensors and Bioelectronics, 2014, 55, 174-179.

10. Xiaohui Lu, Shuo Shi*, Junliang Yao, Xing Gao, Hailiang Huang, Tianming Yao*, Two structurally analogous ruthenium complexes as naked-eye and reversible molecular “light switch” for G-quadruplex DNA, J.Inorg.Biochem., 2014, 140, 64-71.

11. Junliang Yao, Xing Gao, Wenliang Sun, Tianming Yao*, Shuo Shi* and Liangnian Ji, Molecular hairpin: a possible model for inhibition of tau aggregation by tannic acid, Biochemistry-US, 2013,52, 1893-1902.

12. Jun-Liang Yao, Xing Gao, Wenliang Sun, Shuo Shi*, Tian-Ming Yao*, [Ru(bpy)₂dppz-idzo]²⁺: A colorimetric molecular “light switch” and powerful stabilizer for G-quadruplex DNA, Dalton Trans., 2013, 42, 5661.

13. Yao Junliang, Gao Xing, Sun Wenliang, Fan Xuezhong, Shi Shuo*, Yao Tianming*, A Naked-Eye On–Off–On Molecular “Light Switch” Based on a Reversible “Conformational Switch” of G-Quadruplex DNA, Inorg.Chem., 2012, 51, 12591.

14. Ting-Ting Cheng, Jun-Liang Yao, Xing Gao, Wenliang Sun, Shuo Shi* and Tian-Ming Yao*, A new fluorescence “switch on” assay for heparin detection by using a functional ruthenium polypyridyl complex, Analyst, 2013, 138, 3483.

15. Wenliang Sun, Junliang Yao, Tianming Yao* and Shuo Shi*, Label-free fluorescent DNA sensor for the detection of silver ions based on molecular light switch Ru complex and unmodified quantum dots, Analyst, 2013, 138, 421.

16. Dan-Jing Yang, Shuo Shi*, Tian-Ming Yao*, Liang-Nian Ji, Cooperative folding of tau peptide by coordination of group IIB metal cations during heparin-induced aggregation, Biometals, 2012, 25, 361-372.

17. Wenliang Sun, Tianming Yao* and Shuo Shi*, A molecular light switch Ru complex and quantum dots for the label-free, aptamer-based detection of thrombin, Analyst, 2012, 137, 1550–1552.

18. Dan-Jing Yang, Shuo Shi, Tian-Ming Yao*, and Liang-Nian Ji, The Impacts of Hg(II) Tightly Binding on the Alzheimer’s Tau Construct R3: Misfolding and Aggregation, Bull.Chem. Soc. Jpn., 2011,84, 1362-1367.

19. Dan-Jing Yang, Shuo Shi, Leng-Feng Zheng, Tian-Ming Yao*, and Liang-Nian Ji , Mercury(II) Promotes the in vitro Aggregation of Tau Fragment Corresponding to the Second Repeat of Microtubule-Binding Domain: Coordination and Conformational Transition, Biopolymers, 2010,93 1100.

20. Ling-Feng Jiang, Tian-Ming Yao*, Zhi-Liang Zhu, Liang-Nian Ji, “Impacts of Cd(II) on the conformation and self-aggregation of Alzheimer’s tau fragment corresponding to the third repeat of microtubule-binding domain” Biochimica et Biophysica Acta, 2007，1774:1414-1421.

21. Koji Tomoo, Tian-Ming Yao*, Toshimasa Ishida, et al. " Possible Role of Each Repeat Structure of the Microtubule-Binding Domain of the Tau Protein in In Vitro Aggregation", Journal of Biochemistry, 2005, 138: 413-423.

22. Katsuhiko Minoura, Tian-Ming Yao*, Koji Tomoo, Toshimasa Ishida, et al. "Different associational and conformational behaviors between the second and third repeat fragments in tau microtubule-binding domain", European Journal of Biochemistry, 2004, 271: 545-552.

23. Shuko Hiraoka, Tian-Ming Yao, Koji Tomoo, Toshimasa Ishida, et al, "Conformational Transition State Is Responsible for Assembly of Microtuble-binding Domain of Tau Protein", Biochem Biophys Res Commun. 2004, 315(3): 659-663.

24. Tian-Ming Yao*,Koji Tomoo, Toshimasa Ishida, et al. "Aggregation Analysis of the Microtubule Binding Domain in Tau Protein by Spectroscopic Methods", Journal of Biochemistry, 2003, 134: 91-99.