田志新 教授

个人简介

1997年于湖南师范大学化学系获得化学本科学位，2000年于湖南师范大学化学系获得化学硕士学位，2003年于中国科学院化学研究所分子反应动力学国家重点实验室获得化学博士学位。2004-2011年先后在明尼苏达大学化学系和太平洋西北国家实验室从事合作研究。2011年被聘为中国科学院大连化学物理研究所研究员，高分辨质谱技术研究组组长。2013年被聘为同济大学化学化学科学与工程学院教授。

目前主要研究领域是基于生物质谱的蛋白质组学与糖蛋白质组学。发明了生物质谱原位解析算法“同位素质荷比及轮廓指纹比对，iMEF”，并基于iMEF算法发展了完整蛋白质数据库搜索引擎ProteinGoggle、磷酸化修饰定位工具P-bracket、N-连接糖数据库搜索引擎GlySeeker、完整N-糖肽数据库搜索引擎GPSeeker；在top-down蛋白质组学（数据库搜索、同位素标记定量分析），磷酸化蛋白质组学（位点准确定位），糖组学（数据库搜索、大规模鉴定与视图）等研究领域取得了一定的研究成果。在Chemical Communications, Analytical Chemistry, Analytica Chimica Acta, Analytical and Bioanalytical Chemistry, Journal of Proteomics, Scientific Reports, Chemical Reviews等刊物上发表论文60多篇，申请发明专利10多项，获发明专利授权6项及软件著作权1项。

研究方向：

1.蛋白质质谱新技术新方法

2.位点和结构特异定量糖蛋白质组学新技术新方法

3.癌症及癌症干细胞N-糖蛋白标志物的发现及验证

主要科研项目：

1.国家自然科学基金面上项目， 基于近同重二甲基标记和同位素轮廓指纹比对的完整N-糖肽定性定量分析研究（主持，21775110，直接费用65.00万元， 2018-2021）

2.国家自然科学基金面上项目， 基于同位素轮廓指纹比对和同重二甲基标记的整体蛋白质组定量研究（主持，21575104，直接费用65.00万元， 2016-2019）

近3年研究论文与综述：

1.Kaijie Xiao, Zhixin Tian*. Site- and structure-specific quantitative N-glycoproteomics using RPLC-pentaHILIC separation and intact N-glycopeptide search engine GPSeeker. Current Protocols in Protein Science, invited protocol, submitted.

2.Feifei Xu#, Yue Wang#, Kaijie Xiao, Yun Chen*, Zhixin Tian*. Quantitative site- and structure-specific N-glycoproteomics characterization of differential N-glycosylation in ADR-treated MCF7 cancer stem cells. 2019, submitted.

3.Yue Wang, Kaijie Xiao, Zhixin Tian*. Quantitative N-glycoproteomics using stable isotopic diethyl labeling. 2019, submitted.

4.Kaijie Xiao, Yuyin Han, Hailun Yang, Haoran Lu, Zhixin Tian*. Mass spectrometry-based qualitative and quantitative N-glycomics: an update of 2017-2018. Analytica Chimica Acta, invited review, 2019, submitted.

5.Yue Wang#, Feifei Xu#, Kaijie Xiao, Yun Chen* and Zhixin Tian*. Site- and structure-specific characterization of N-glycoprotein markers of MCF-7 cancer stem cells using isotopic-labeling quantitative N-glycoproteomics. Chemical Communications, 2019, 55, 7934—7937.

6.Kaijie Xiao, Zhixin Tian*. GPSeeker enables quantitative structural N-glycoproteomics for site- and structure-specific characterization of differentially expressed N-glycosylation in hepatocellular carcinoma. Journal of Proteome Research, 2019, 10.1021/acs.jproteome.9b00191.

7.Yun Shen#, Yiwen You#, Kaijie Xiao, Yun Chen*, Zhixin Tian*. Large-scale identification and fragmentation pathways analysis of N-glycans from mouse brain. Journal of American Society for Mass Spectrometry, 2019, DOI: 10.1007/s13361-019-02181-y.

8.Bingbing Xue, Kaijie Xiao, Zhixin Tian*. Top-down characterization of mouse core histones. Journal of Mass Spectrometry, 2019, 54, 258-265.

9.Yuyin Han, Kaijie Xiao, Zhixin Tian*. Comparative glycomics study of cell-surface N-glycomes of HepG2 versus LO2 cell lines. Journal of Proteome Research, 2019, 18(1),372-378.

10.Hao Wang, Zhixin Tian*. Facile synthesis of titanium (IV) ion immobilized poly-glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific intact phosphoproteins enrichment. Journal of Separation Science, 2018, 41, 4194-4202.

11.Shasha Li#, Kaijie Xiao#, Yue Zhou#, Jing Li*, Zhixin Tian*. Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid Mass Spectrometer. Rapid Communications in Mass Spectrometry, 2018, 32, 2031-2039.

12.Hao Wu, Kaijie Xiao, Zhixin Tian*. Top-down characterization of chicken core histones. Journal of Proteomics, 2018, 184, 34-38.

13.Hao Wang, Zhixin Tian*. Facile synthesis of titanium (IV) ion immobilized adenosine triphosphate functionalized silica nanoparticles for highly specific enrichment and analysis of intact phosphoproteins. Journal of Chromatography A, 2018, 1564, 69-75.

14.Kaijie Xiao, Yuyin Han, Zhixin Tian*. Large-scale identification and visualization of human liver N-glycome enriched from LO2 cells. Analytical and Bioanalytical Chemistry, 2018, 410 (17), 4195–4202.

15.Hao Wang, Jinqiang Zhong, Kaijie Xiao, Zhixin Tian*. Enrichment of intact phosphoproteins using immobilized titanium (IV) affinity chromatography microspheres. Separation Science Plus, 2018, 1(2), 93-99.

16.Kaijie Xiao, Yue Wang, Yun Shen, Yuyin Han, Zhixin Tian*. Large-scale identification and visualization of N-glycans with primary structures using GlySeeker. Rapid Communications in Mass Spectrometry, 2018, 32, 142-148.

17.Kaijie Xiao, Yun Shen, Shasha Li, Zhixin Tian*. Accurate Phosphorylation Site Localization Using Phospho-brackets. Analytica Chimica Acta, 2017, 996, 38-47.

18.Kaijie Xiao, Fan Yu, Houqin Fang, Bingbing Xue, Yan Liu, Yunhui Li, and Zhixin Tian*. Are neutral loss and internal product ions useful for top-down protein identification? Journal of Proteomics, 2017, 160, 21-27.

19.Kaijie Xiao, Fan Yu, Zhixin Tian*. Top-down Protein Identification Using Isotopic Envelope Fingerprinting. Journal of Proteomics, 2017, 152, 41-47.

20.肖开捷，田志新*。整体蛋白质电喷雾质谱原位解析。生命的化学，2017，37(1)，16-21。

“生物分析与质谱”课题组热诚欢迎具有化学、计算机科学、生命科学、医学等背景的学生的加入！”